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The Physiology of Micro-Aerobic Fermentation

Conventional composting uses air to slowly combust organic materials.  This results in the emission of large quantities of GHGs into the atmosphere which, in combination with the combustion of fuel used in processing waste materials, contributes to global climate change.  It is also labor intensive and costly to operate on a large scale. Its net impact on the environment is negative - more is taken from the environment by this processing method than is returned.  Less than 50% of the carbon composted gets returned to the soil.  We are studying more eco-friendly low-carbon footprint bio-technologies for processing recyclable vegetal materials. 

Micro-Aerobic Composting  is the green alternative to conventional composting, with the potential of recovering up to 2/3rds of carbon in forms that can be returned back into soil ecosystems.  In Micro-Aerobic Composting vegetal materials of Types 1 and 3 materials (vegetative waste and mixed food waste) are broken down rapidly; this process is less labor- and energy-intensive, and gives up much less GHGs to the atmosphere relative to conventional composting.  Micro-Aerobic Composting is suitable for residential applications, (where it leads to substantial savings in garbage pickup costs), but can also be scaled-up for farm-level composting.  This biotechnology creates synergy between: cities (needing to lower garbage hauling and processing costs); farmers (looking for safe and low cost fertilizers); and urban population (with increasing demand for organic produce). 
 

The steps of Micro-Aerobic Composting  

• Step 1. Isolation of microbes giving the best ADC performance.  These microbes are induced to make spores and can be stored dried or frozen.  This step is done in microbiology labs and rich cultures are then made available to users.

• Step 2. Micro-Aerobic Fermentation, takes about 1-3 weeks (depending on materials and conditions) and leads to partially degraded cellulose (with very little CO2 and zero methane production).

• Step 3. Decomposition in soil.  The acidity of the fermented product is first buffered and the mixture is finally mixed into soil where it will fully decompose in about one year and becomes part of the ecosystem. About one month after mixing the compost material into soil the soil can be planted.

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AFC RECIPES

1) AFC using food scraps, leaves and grass clippings

AFC should not be confused with conventional composting.  In conventional composting a random mixture of organic materials of various types and sizes are stored in a bin, pile or composting box.  Given enough time (this may last anywhere from six months to more than a year), and with the help of microbial oxidation a slow degradation will occur.  Disadvantages of conventional composting include the long time needed to obtain a stable end product, high processing costs (in commercial composting) for turning over the decaying waste so that it is properly aerated, bad smells, the generation of large quantities of methane and carbon dioxide (two potent greenhouse gases), and loss of a significant portion of energy and organic carbon in the process.  AFC is a controlled two step microbial biotechnology that eliminates these problems, though to be successful proper raw materials, bacteria, and procedures must be observed.  

AFC and recyclable materials  

The amendments added to the overall mixture vary based on the proportion between the different start materials.  The recipe given here is adjusted for mixtures of kitchen vegetable scraps, leaves, grass clippings and very small amounts (< 5 %) of shredded paper.  Egg shells can also be added, but not the eggs content.   For decomposing mixtures rich in paper, cardboard boxes, hay, straws, mulch and sawdust a different recipe is needed.  DO NOT USE animal waste, any significant amounts of oil, bones, meat, plastics, metals, unshredded wood or cloth (except for pure shredded cotton).  Because ADC is based on active microbes chemical household products that may be toxic and inhibit the growth of bacteria (such as detergents, soap, paint, gasoline, oil, antifreeze, litter box contents, etc.) should be avoided. 

Fragment size

Raw materials used in AFC have to be fragmented into small pieces.  Crumbling or cutting up food waste into bite size pieces works very well.  Tearing a piece of bread into two or three pieces also works well.  For a whole melon, apple, cucumber, or other spoiled fruit or vegetable, cutting up the produce into strips or sections as if it were to be served on a plate is recommended.  For paper, or leaves, a small wood chopper or paper shredder makes excellent size fragments.  If a wood chopper is not available, leaves and grass will fragment easier if they are dried first, then torn or broken up into small fragments.  For shorter fermentation it is highly recommended for grass clippings to be dried first.  Paper coffee filters do not need to be shredded because they are already very thin and porous and will degrade without difficulty, and can be processed right along with coffee grounds held inside the filters.

The fermentor  

The fermentation step of AFC occurs in a bucket or drum called a fermentor.  Fermentors have an internal lid that rests on the mixture (see the figure above).  Do not allow too much space between the internal lid and the sides of the fermentor (¼ of an inch or ~0.5 cm space is acceptable).  AFC fermentors are designed to control the access of air to the fermenting mixture.  If a garbage bin is used to construct a fermentor, make certain to also include an inner lid for closing off access of the air space inside the bin.  The fermentation step is anaerobic (or at most microaerobic).  This means that it occurs in the absence of air, or at the very least it must occur at very low concentrations of oxygen.  AFC fermentors need to be drained.  During fermentation liquid will accumulate in the fermentor and percolate to the bottom.  This liquid should not be allowed to accumulate for lengthy periods.  It will inhibit the fermentation.  A small drain for stopping and starting drainage is placed at the bottom of the fermentor.  Using this system, the fermentor can be periodically “bled” of excess liquid as it accumulates.  Draining the small amount of fluid that accumulates (about a cup per day in a 5 gallon bucket) every other day ensures efficient operation of the fermentor.  Alternatively, if there is a convenient place to continuously drain the fermentor, that works, too.  The fluid can be safely flushed down the drain, or diluted and used as liquid fertilizer. Some internet providers sell customized fermentors (called Bokashi fermentors) of different sizes.  An improvisation made from a garbage bin also works well, but in this case a drainage hole has to be made at the lowest point in the bin.  The inner lid should not be very heavy to avoid compaction.  It can be improvised from wood, plywood, plastic or aluminum, or ceramic materials.  Very large, especially tall, garbage bins with the capacity of holding ~60 gallons (~250 liters) are too heavy to manipulate.  They will also show compaction toward the bottom, and give poorer results. 

STEP 1. OBTAINING THE STARTER BACTERIA 

A starter has to be used containing microbes with high AFC efficiency.  In research laboratories this is done by selecting bacteria from nature.  Some Bokashi providers offer a mixtures of microbes and fermented cellulose called “effective microorganisms”.  Some people use wheat bran as a starting inoculum.  Wheat bran is (in most cases) rich in spores of microbes from soil including Clostridium, which are important in the fermentation step.   

STEP 2. FERMENTATION  

• Add a grid at the bottom of the fermentor.  This is very important to allow the extra water formed during fermentation to drain. 

The preparation depends on composition.

• If the raw fermentable materials are dominated by kitchen waste start adding layers of fermentable materials in the fermentor, lightly dust them with the starter bacteria (wheat bran or the “effective microorganisms powder”).  Some shredded paper can also be added but no more than 5 %.  

• If the mixture is dominated by grass clippings, leaves and shredded paper, apart from the starter the mixture should be sprayed with a solution of ~1 % molasses dissolved in water.  (Alternatively sugar can be used at a final concentration of about 1 gram per liter; this means one spoon of sugar to every 10 liters or ~ 2.5 gallons of water).  It is better for the grass clippings and leaves to be dried before they are used.   

•Add 5 g of sea salt to each 10 L of water (this is approximately one teaspoon of salt to every 10 liters or ~2.5 gallons).  

• Do not compact the mixture in the fermentor.  The mixture should be moist and fluffy, with lots of gas spaces. 

• Add the inner lid and allow it to rest on top of the fermenting waste.

• Cover with the top lid.  

•As more materials need to be fermented keep on adding them under the inner lid and treat them with the starter, molasses, salt, as shown above.  Avoid exposing the fermenting mixture to air for extensive periods of time.  

• When one fermentor is filled keep it closed for 2-6 weeks to complete the fermentation; meanwhile begin filling a second fermentor.  In a typical household setting two fermentors of 5 gallons each works well.   

• Avoid too much water.  If excessive water is present the fermentation will slow down, acidity develops slower, methane and hydrogen sulfide could form, and the fermented product begins to stink.  Bad odors are also a sign of improper raw materials in the initial mixture.    

Incubation conditions 

Fermentation starts very fast (two or three days).  Low temperature slows this process.  Do not allow the fermentor’s content to freeze – it will stop the activity of the microbes.  Do not expose the fermentor to high heat.  For households, the best place to store the fermentor is inside the house in a convenient location such as the kitchen, or in a pantry or garage where access is easy and it can be drained and serviced with little effort.  On a farm in the summer the best place to place the fermentor is in the shade.  In winter, a garage or a tool shed are best. 

The evolution of fermentation 

In early steps some microbes from the community use the sugar (or molasses) and consume most of the oxygen (but not all).  Then bacteria called clostridia germinate from spores and start fermenting the remaining sugar (or molasses) while producing small organic acids.  This acidity (in combination with the low oxygen concentration) inhibits other microbes and allows clostridia to become dominant.  When the concentration of sugars is near zero (generally after a couple of days), the Clostridium microbes start producing enzymes called cellulases.  These enzymes degrade the cellulose into smaller fragments and make more sugar available to the microbes.  Monitor the evolution of fermentation.  The mixture should have a sweet vinegary fruity odor.  If the mixture starts to stink the fermentation is not working correctly (this may mean too much water, animal products, wrong acidity, wrong compaction, wrong microbes, etc).  Stop immediately and bury.

Draining  

During fermentation liquid forms and drains toward the bottom.  This liquid is acidic, very rich in organic carbon and in energy and should not be used directly to water plants with it.  Similar to other nutrient-rich liquid fertilizers, because it is very concentrated it may kill plant roots if added directly to the soil.  Dilute it first about ten times in water. 

STEP 3. DECOMPOSITION  

Final decomposition occurs in mixtures with soil.  The fermented materials produced in Step 2 (see figure) will preserve their general shape and size, but inside these materials the degradation of cellulose is advanced already.  This fermented mixture cannot be used as compost; it is too concentrated, and the decomposition is not yet finished.  

• Dig a hole in the ground about 0.5-1 foot deep (~15-30 cm) and add an amount of fermented material.  Add loose soil to obtain a about 50/50 mixture and mix with a spade or shovel. 

• Cover up the mixture with loose soil and leave to decompose for at least one week.

While in the soil other microorganisms will start degrading the smaller organic molecules and then continue with the partly degraded cellulose.  

Fertilization with the AFC product

After a week or two in the soil decomposition is almost finished and the mixture can be used directly as a fertilizer in flower beds, garden, or added at the root of trees.  Remember that this is a carbon recycling method.  Because it is rich in organic carbon this fertilizer is most efficient if it is incorporated in soil rather than added on top.  Alternatively, the soil-fertilizer mixture can be stored as a compost pile or dried, bagged and used later.  This mixture is very rich in nutrients and energy and will have a strong positive effect on plant growth.  

On average about 25-30 % of a household’s garbage can be treated by AFC.  In gardens this percentage will be higher, because leaves and grass are often produced in larger amounts. One great benefit is that the materials used in AFC are recycled instead of being thrown away as garbage.


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